Goal 1: Promote Human Health

Adult Cardiomyocytes in Culture

So much basic cardiovascular discovery relies on cell culture models. While cardiac cell lines exist (e.g. HL-1, H9c2), these often poorly model aspects of cardiomyocyte function in-situ (e.g. contractile function, metabolism). In contrast, primary cardiomyocytes isolated from adult animals (especially mice!) are not readily amenable to culture conditions. Even if cells can be kept alive, they are often refractory to genetic manipulations (e.g. transfection). CHALLENGE: Can simple protocols be developed, to culture adult cardiomyocytes, permitting their genetic manipulation over the course of several days/weeks?

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Idea No. 355