Goal 2: Reduce Human Disease

Develop and apply single-cell-based assays to verify/modify existing biological theories

Many biological models developed to date are based on studies using heterogeneous cell populations. For example, ChIP-Seq studies have shown that RNA Pol II is frequently paused near promotes and that in stem cells, developmentally poised genes are controlled by bivalent enhancers. These studies are largely based on statistical analyses of average ChIP-Seq reads from millions of cells. Earlier biochemical analysis of transcriptional mechanisms is also based on purified factors derived from heterogeneous cell populations. A concern is that these results may not faithfully represent what happens in a single cell. Therefore, it will be important to verify, and possibly modify, the existing theories by the emerging single-cell-based assays.

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Idea No. 1052