Emerging importance of RNA regulation
What are the roles of RNAs (e.g., microRNAs, long non-coding RNAs, etc.) in myocardial growth, adaptation, and injury healing response?
What are the roles of RNAs (e.g., microRNAs, long non-coding RNAs, etc.) in myocardial growth, adaptation, and injury healing response?
What specific roles do protein glycosylation and protein complex formation on stable and activated cellular membranes play in inherent immunogenicity and antibody formation to life-saving therapies for rare blood disorders?
NHLBI leadership and collaboration with NIAID is needed to bring this combined expertise to bear on the understanding and prevention of this most significant complication of hemophilia therapy.
What is the level of population variation of gene regulatory networks in heart, lung, and blood and sleep disease mechanisms? Can we use different animal model strains to identify key factors in networks and modifier genes?
What is the interplay of the micro- and macroenvironment and their effect on gene and protein expression and overall effect on phenotypes in HLBS systems?
Need for unbiased approaches for information capture. Technologies for capturing real time, dynamic and contextual information
What are the regional variations in cellular and molecular characteristics (from epigenetics to microbiome) in the lung, and what controls these variations?
Is there a critical window of growth and development for maximizing lung function?
How do lung progenitors recognize stem cell niches, and what cell-cell interactions mediate normal repair?
What are the molecular and cellular responses in the lung that occur after environmental stimuli that predict homeostatic resilience or transition to disease, disorder, or aging?
What is the best method to develop an integrated map of the expression patterns of the vasculature (RNA, protein, and epigenome) within each organ system?
Sampling standards and quality control are major outstanding issues.
What is the level of intra-tissue variation of cardiac development gene regulatory networks at single cell resolution?
To eventually modulate pathologic communication processes employing new therapeutics, there is a need to better understand the transcellular communication between blood cells and components of the vascular layer in vivo.